MersV1, Main, Exploration, bibRecord, 002F20

Base pair opening in three DNA-unwinding elements.

Identifieur interne : 002F20 ( Main/Exploration ); précédent : 002F19; suivant : 002F21

Base pair opening in three DNA-unwinding elements.

Auteurs : Daniel Coman [États-Unis] ; Irina M. Russu

Source :

The Journal of biological chemistry [ 0021-9258 ] ; 2005.

RBID : pubmed:15784615

Descripteurs français

KwdFr :
- ADN bactérien (), Ammoniac (analyse), Appariement de bases (génétique), Cinétique, Conformation d'acide nucléique, Données de séquences moléculaires, Escherichia coli (génétique), Protons, Réplication de l'ADN, Spectroscopie par résonance magnétique, Séquence conservée, Séquence nucléotidique.
MESH :
- analyse : Ammoniac.
- génétique : Appariement de bases, Escherichia coli.
- ADN bactérien, Cinétique, Conformation d'acide nucléique, Données de séquences moléculaires, Protons, Réplication de l'ADN, Spectroscopie par résonance magnétique, Séquence conservée, Séquence nucléotidique.

English descriptors

KwdEn :
- Ammonia (analysis), Base Pairing (genetics), Base Sequence, Conserved Sequence, DNA Replication, DNA, Bacterial (chemistry), Escherichia coli (genetics), Kinetics, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Nucleic Acid Conformation, Protons.
MESH :
- chemical , analysis : Ammonia.
- chemical , chemistry : DNA, Bacterial.
- genetics : Base Pairing, Escherichia coli.
- Base Sequence, Conserved Sequence, DNA Replication, Kinetics, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Nucleic Acid Conformation, Protons.

Abstract

DNA-unwinding elements are specific base sequences that are located in the origin of DNA replication where they provide the start point for strand separation and unwinding of the DNA double helix. In the present work we have obtained the first characterization of the opening of individual base pairs in DNA-unwinding elements. The three DNA molecules investigated reproduce the 13-mer DNA-unwinding elements present in the Escherichia coli chromosome. The base sequences of the three 13-mers are conserved in the origins of replication of enteric bacterial chromosomes. The exchange of imino protons with solvent protons was measured for each DNA as a function of the concentration of exchange catalyst using nuclear magnetic resonance spectroscopy. The exchange rates provided the rates and the equilibrium constants for opening of individual base pairs in each DNA at 20 degrees C. The results reveal that the kinetics and energetics of the opening reactions for AT/TA base pairs are different in the three DNA-unwinding elements due to long range effects of the base sequence. These differences encompass the AT/TA base pairs that are conserved in various bacterial genomes. Furthermore, a qualitative correlation is observed between the kinetics and energetics of opening of AT/TA base pairs and the location of the corresponding DNA-unwinding element in the origin of DNA replication.

DOI: 10.1074/jbc.M502773200
PubMed: 15784615

Affiliations:

États-Unis

Le document en format XML

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<wicri:regionArea>Department of Chemistry and the Molecular Biophysics Program, Wesleyan University, Middletown, Connecticut 06459</wicri:regionArea>
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<author><name sortKey="Russu, Irina M" sort="Russu, Irina M" uniqKey="Russu I" first="Irina M" last="Russu">Irina M. Russu</name>
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<series><title level="j">The Journal of biological chemistry</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ammonia (analysis)</term>
<term>Base Pairing (genetics)</term>
<term>Base Sequence</term>
<term>Conserved Sequence</term>
<term>DNA Replication</term>
<term>DNA, Bacterial (chemistry)</term>
<term>Escherichia coli (genetics)</term>
<term>Kinetics</term>
<term>Magnetic Resonance Spectroscopy</term>
<term>Molecular Sequence Data</term>
<term>Nucleic Acid Conformation</term>
<term>Protons</term>
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<term>Ammoniac (analyse)</term>
<term>Appariement de bases (génétique)</term>
<term>Cinétique</term>
<term>Conformation d'acide nucléique</term>
<term>Données de séquences moléculaires</term>
<term>Escherichia coli (génétique)</term>
<term>Protons</term>
<term>Réplication de l'ADN</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Séquence conservée</term>
<term>Séquence nucléotidique</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Ammonia</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Ammoniac</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Base Pairing</term>
<term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Appariement de bases</term>
<term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Conserved Sequence</term>
<term>DNA Replication</term>
<term>Kinetics</term>
<term>Magnetic Resonance Spectroscopy</term>
<term>Molecular Sequence Data</term>
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<term>Conformation d'acide nucléique</term>
<term>Données de séquences moléculaires</term>
<term>Protons</term>
<term>Réplication de l'ADN</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Séquence conservée</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">DNA-unwinding elements are specific base sequences that are located in the origin of DNA replication where they provide the start point for strand separation and unwinding of the DNA double helix. In the present work we have obtained the first characterization of the opening of individual base pairs in DNA-unwinding elements. The three DNA molecules investigated reproduce the 13-mer DNA-unwinding elements present in the Escherichia coli chromosome. The base sequences of the three 13-mers are conserved in the origins of replication of enteric bacterial chromosomes. The exchange of imino protons with solvent protons was measured for each DNA as a function of the concentration of exchange catalyst using nuclear magnetic resonance spectroscopy. The exchange rates provided the rates and the equilibrium constants for opening of individual base pairs in each DNA at 20 degrees C. The results reveal that the kinetics and energetics of the opening reactions for AT/TA base pairs are different in the three DNA-unwinding elements due to long range effects of the base sequence. These differences encompass the AT/TA base pairs that are conserved in various bacterial genomes. Furthermore, a qualitative correlation is observed between the kinetics and energetics of opening of AT/TA base pairs and the location of the corresponding DNA-unwinding element in the origin of DNA replication.</div>
</front>
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<affiliations><list><country><li>États-Unis</li>
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Serveur d'exploration MERS

Base pair opening in three DNA-unwinding elements.

Base pair opening in three DNA-unwinding elements.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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